Generator

Part:BBa_J100105:Design

Designed by: Erin McGuire   Group: Campbell M Lab   (2013-01-29)


Low production of eCDM8


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 3275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3276
    Illegal PstI site found at 3290
    Illegal NotI site found at 3283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 237
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 3276
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 3276
    Illegal PstI site found at 3290
    Illegal AgeI site found at 196
    Illegal AgeI site found at 857
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

S05008 was ligated using Bsa1, so there is no scar between the two parts and Pst1.


Source

eCDM8 was synthesized de novo.


References